| Description: |
In total, five replicates of 495 genotypes were surveyed in this experiment (435 hybrid genotypes, 30 parental genotypes from manual crosses, and 30 self-fertilized parental genotypes). Five unsterilized seeds for each replicate were aliquoted into 1.5-mL tubes with 500 μL of ddH20. Seeds were stratified in the dark at 4 °C for 10 days. After stratification, seeds were sown into soil (CL T Topferde; www.einheitserde.de) pots in a completely randomized design. Flats were covered with humidity domes and placed into 16 °C growth chambers under long-day conditions (16 hours light: 8 hours dark) at a relative humidity of 65%. Light bulbs were a mixture of Sylvania Cool White Deluxe to Warm White Deluxe fluorescent bulbs (4:2) (www.havells-sylvania.com/en-GB/sylvania). Humidity domes were removed after 1 week, and pots were manually thinned to one plant per pot. |
