| Phenotype ID: |
AT1P25078 |
| Name: |
area 21d protocol a |
| Species: |
Arabidopsis thaliana
|
| Dataset: |
1001 Genomes Data
|
| Variation Type: |
continuous |
| Number of accessions: |
129
|
| Category: |
|
| Phenotype scoring: |
Area of root explants after 21 days on shoot induction medium (SIM; B5 supplemented with 25 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid) following protocol a (see description) |
| Source: |
Danny Geelen and Robin Lardon, HortiCell, Department of Plants and Crops, Ghent University, Ghent, Belgium. |
| Description: |
The area was calculated automatically in ImageJ by applying a colour threshold to distinguish explants from the background and subtracting the latter to obtain a 2D projection as a proxy for the regenerative potential.
Protocol a: 7-mm-long apical root segments were excised from 7-day-old seedlings, grown under cool white fluorescent tungsten tubes (70 µM m^-2 s^-1; 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to SIM. |
