| Phenotype ID: |
AT1P24065 |
| Name: |
shoots 15d protocol a |
| Species: |
Arabidopsis thaliana
|
| Dataset: |
1001 Genomes Data
|
| Variation Type: |
continuous |
| Number of accessions: |
130
|
| Category: |
|
| Phenotype scoring: |
Manual count of regenerated shoots from root explants after 15 days on shoot induction medium (SIM; B5 supplemented with 25 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid) following protocol a (see description). |
| Source: |
Danny Geelen and Robin Lardon, HortiCell, Department of Plants and Crops, Ghent University, Ghent, Belgium. |
| Description: |
Shoots were defined as leaf-like structures with strong greening, rounded edges and often covered with trichomes.
Protocol a: -mm-long apical root segments were excised from 7-day-old seedlings, grown under cool white fluorescent tungsten tubes (70 µM m^(-2) s^(-1); 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to SIM. |
