General information

Phenotype ID: AT1P23868
Name: shoots 21d protocol a
Species: Arabidopsis thaliana
Dataset: 1001 Genomes Data
Variation Type: continuous
Number of accessions: 129
Phenotype scoring: Manual count of regenerated shoots from root explants after 21 days on shoot induction medium (SIM; B5 supplemented with 25 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid) following protocol a (see description)
Source: Danny Geelen and Robin Lardon, HortiCell, Department of Plants and Crops, Ghent University, Ghent, Belgium.
Description: Shoots were defined as leaf-like structures with strong greening, rounded edges and often covered with trichomes. Protocol a: 7-mm-long apical root segments were excised from 7-day-old seedlings, grown under cool white fluorescent tungsten tubes (70 µM m-2 s-1; 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to SIM.

Additional Meta Information

No additional meta information available

Phenotype Distribution


Shapiro-Wilk Test Statistic

The Shapiro-Wilk-Test tests the null hypothesis that the data was drawn from a normal distribution.



authorstitlepub yearjournalvolumepagesdoi
Robin Lardon et al.The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors2020Communications Biology3N-A10.1038/s42003-020-01274-9