| Description: |
Tocopherols were extracted from 10-12 mg of mature, dry Arabidopsis seeds as described in Gonzalez-Jorge et al. 2013 (10.1105/tpc.113.119677). Individual compounds were resolved by chromatography at 30° on a 3-mm × 100-mm YMC C30 column with a 3-μm particle size (Waters, Milford, MA). Details of the HPLC mobile phases and flow rate are present in Lipka et al. 2013 (10.1534/g3.113.006148). Tocopherols were detected by fluorescence using 290 nm excitation and 325 nm emission and quantified relative to calibration curves with γ-, α-, and δ-tocopherol with commercial standards (Supelco). |
